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PGE2 EIA Kit

$310.00
SKU
EA02
Product Overview

Background


Prostaglandin E2 (PGE2) is derived from Prostaglandin H2 (PGH2), which in turn is synthesized from arachidonic acid, through the cyclooxygenase pathway. Many cell types, such as, epithelial cells, fibroblasts, and macrophages produce PGE2. PGE2 possesses vasoactivity, modulates immune functions, regulates sleep-wake cycle and exhibits many other activities. This competitive ELISA kit provides quantitative analysis of PGE2 levels in biological fluids based on the competition between the PGE2 present in the sample and PGE2-enzyme conjugate for a limited number of antibody binding sites.

 

 

Assay Principle


This is an ELISA for the quantitative analysis of PGE2 levels in biological fluid.  This test kit operates on the basis of competition between the enzyme conjugate and the PGE2 in the sample for a limited number of binding sites on the antibody coated plate.

The sample or standard solution is first added to the microplate.  Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour.  During the incubation, competition for binding sites is taking place.  The plate is then washed removing all the unbound material.  The bound enzyme conjugate is detected by the addition of substrate which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 450nm or 650nm.  The extent of color development is inversely proportional to the amount of PGE2 in the sample or standard.  For example, the absence of PGE2 in the sample will result in a bright blue color, whereas the presence of PGE2 will result in decreased or no color development.

If using urine as a sample it is recommended to run our

 

 

Creatinine Assay kit (CR01)

in conjunction with your samples since urinary creatinine levels may be used to normalize the rate of excretion of other analytes.
PGE2,PGE2 ELISA,PGE2 assay

Cross Reactivity


Prostaglandin E2
Prostaglandin A1
Prostaglandin A2
Prostaglandin B1
Prostaglandin B2
Prostaglandin E1
6-keto-prostaglandin E1
Prostaglandin E3
Prostaglandin F1α
13, 14-dihydro-15-keto-prostaglandin F2α
Prostaglandin D2
11b-prostaglandin F2α
6-keto-prostaglandin F1α
Leukotriene B4
15-keto-prostaglandin F2α
Prostaglandin F2α
11-dehydro-thromboxane B2
Thromboxane B2

100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
85.50%
17.70%
10.40%
2.00%
0.26%
0.11%
0.10%
<0.01%
<0.01%
<0.01%
<0.01%
<0.01%

 

Spec Sheet