Are you getting the most out of your antioxidant analyses?
Oxford Biomedical’s Total Antioxidant Capacity assay is based on the CUPRAC method originally developed by our collaborator Dr. Resat Apak and his colleagues at the University of Istanbul. The reduction of copper-cuprione complexes by radical scavenging antioxidants is well-established, and is more convenient and typically more reliable than several traditional assays (e.g. TRAP, FRAP, etc). The CUPRAC method (1,2) is based on the reduction of copper-Neocuprione complex by radical scavenging antioxidants and reacts well with both hydrophilic and lipophilic antioxidants. In contrast, methods that employ a copper-bathocuprione (BCS) complex (3) have multiple drawbacks, especially for measuring the antioxidant capacity of lipophilic antioxidants (4).
Our CUPRAC antioxidant capacity assay exhibits distinctly stronger responses than cupric-BCS to naringenin, a-tocopherol, and bilirubin. Dr. Apak and colleagues recent demonstrated that the antioxidant capacity due to beta-carotene (in acetone or dichloromethane) was fully measurable by conventional CUPRAC but not at all by cupric-BCS assay (4). A list of several of the most recent and most relevant publications that employed Oxford’s CUPRAC-based antioxidant power assay kit is provided for your convenience.
NOTE: It may be of interest that Professor Apak was recently awarded the Turkish Scientific and Technological Research Institution science award. The TUBITAK is the highest science award, and is also known as the "Turkish Nobel".
1. RESAT APAK, KUBILAY GU¨ C¸ LU¨ , MUSTAFA O¨ ZYU¨ REK, SALIHA ESI°N KARADEMI°R, & MEHMET ALTUN. Total antioxidant capacity assay of human serum using copper(II)-neocuproine as chromogenic oxidant: The CUPRAC method Free Radical Research, September 2005; 39(9): 949–961
2. Apak, MS, Guclu, K, Ozyurek, M, Bener, M, Martinez, E. and Callewaert, D.M., Optical Sensor Based Cupric Reducing Antioxidant Capacity (CUPAC) Assay, U.S. Patent 8,912,004 issued December 14, 2014
3. Sigma-Aldrich Antioxidant Assay Kit. product CS0790
4. Esin Çelik, Mustafa .zyürek, Kubilay Gü.lü, Res_at Apak Differences in responsivity of original cupric reducing antioxidant capacity and cupric–bathocuproine sulfonate assays to antioxidant compounds. Analytical Biochemistry 423 (2012) 36–38.
Recent and diverse studies that employed Oxford Biomedical’s CUPRAC-based Total Antioxidant Capacity assay kit (TA-02)
F.Affane, F.Bensalah, N.I.Harrat, H.Chekkal, S.Louala, and M.Y.Lamri-Senhadji Sardine purified proteins improve blood pressure, glycemic control, anti-atherogenic metabolic pathways and antioxidant capacity in obese rats. Annales de Cardiologie et d'Angéiologie 67:154-160 (2018)
Fadzel Wong Chee Ping, Chee Keong Chen, Foong Kiew Ooi, and Mahaneem Mohamed. Effects of bee bread supplementation on endurance running performance and total antioxidant status in recreational athletes. Int. J. Applied Res. Nat. Prod. 11:17-23 (2018)
Kunio Yui, Nasoyuki Tanuma, Hiroshi Yamada, and Yohei Kawasaki Decreased total antioxidant capacity has a larger effect size than increased oxidant levels in urine in individuals with autism spectrum disorder. Environmental Science and Pollution Research 24: 9635–9644 (2017)
Masaharu Hayashi, Rie Miyata and Naoyuki Tanuma. Reduced endogenous urinary total antioxidant power and its relation of plasma antioxidant activity of superoxide dismutase in individuals with autism spectrum disorder. Int. J. Developmental Neuroscience 60:405-420 (2017)
Karen L.Pritsos, Cristina R., PerezaThivanka. Muthumalage, Karen M.Dean, DaveCacela. KatieHanson-Dorr. FredCunningham, Steven J.Bursian, Jan E. Link, SusanShriner, KatherineHorake and Chris A.Pritsosa. Dietary intake of Deepwater Horizon oil-injected live food fish by double-crested cormorants resulted in oxidative stress. Ecotoxicology and Environmental Safety 146: 62-67 (2017)
Natarajan Saravanan, Madhoosudhan Ananth Patil, Puthcha Uday Kumar, Palla Suryanarayana, and Geereddy Bhanuprakash Reddy, Dietary ginger improves glucose dysregulation in a long-term high-fat high-fructose fed prediabetic rat model. NISCAIR-CSIR, India. Mar-2017
Juan Carlos Cantón-Romero, Alejandra Guillermina Miranda-Díaz, Jose Luis Bañuelos-Ramírez, Sandra Carrillo-Ibarra, Sonia Sifuentes-Franco, José Alberto Castellanos-González, and Adolfo Daniel Rodríguez-Carrizalez. Markers of Oxidative Stress and Inflammation in Ascites and Plasma in Patients with Platinum-Sensitive, Platinum-Resistant, and Platinum-Refractory Epithelial Ovarian Cancer. Oxidative Medicine and Cellular Longevity. 2017: article ID 2873030
Min-Hae Song, Ha-Na Kim, Yong Lim, and In-Surk Jang. Effects of coenzyme Q10 on the antioxidant system in SD rats exposed to lipopolysaccharide-induced toxicity. Lab Anim Res. 33:24-31 (2017)
Ionan Marigómez , Maria Múgica, Urtzi Izagirre, and Inna M. Sokolova
Chronic environmental stress enhances tolerance to seasonal gradual warming in marine mussels. PLOS-ONE. https://doi.org/10.1371/journal.pone.0174359 (2017)
Hee Jung Park, Myung Ju Lee, and Hye Ran Lee. Effects of temperature-fluctuation in a refrigerator on antioxidative index and storage qualities of various foods. J Nutr Health 50: 133 -141 (2017)
Adolfo Daniel Rodríguez-Carrizalez, José Alberto Castellanos-González, Esaú César Martínez-Romero, Guillermo Miller-Arrevillaga, Fermín Paul Pacheco-Moisés, Luis Miguel Román-Pintos & Alejandra Guillermina Miranda-Díaz. The effect of ubiquinone and combined antioxidant therapy on oxidative stress markers in non-proliferative diabetic retinopathy: A phase IIa, randomized, double-blind, and placebo-controlled study. Redox Report Communications in Free Radical Research. 21: Issue 4 (2016)
Umar Zayyanu Usman, Ainul Bahiyah Abu Bakar, and Mahaneem Mohamed Metformin Reduces Oxidative Stress Status and Improves Plasma Insulin Level in Streptozotocin-Induced Diabetic Rats. Lifescience Global. 6: No 4 (2016)
CristinaCarrasco, Ana BeatrizRodríguez, and José APariente. Effects of melatonin on the oxidative damage and pancreatic antioxidant defenses in cerulein-induced acute pancreatitis in rats. Hepatobiliary & Pancreatic Diseases International 13: 442-446 (2014)
Bridget D. Mathison, Lindsey L. Kimble, Kerrie L. Kaspar, Christina Khoo, and Boon P.Chewa. Consumption of cranberry beverage improved endogenous antioxidant status and protected against bacteria adhesion in healthy humans: a randomized controlled trial. Nutrition Research 34:420-427 (2014)
M. LeticiaCastroa, Melanie J.McConnell, Patries M.Herst. Radiosensitisation by pharmacological ascorbate in glioblastoma multiforme cells, human glial cells, and HUVECs depends on their antioxidant and DNA repair capabilities and is not cancer specific. Free Rad Biol. Med. 74: 200-209 (2014).
Hye Duck Choi, Ji Hae Kim, Min Jung Chang, Yeo Kyu-Youn, Wan Gyoon Shin. Effects of Astaxanthin on Oxidative Stress in Overweight and Obese Adults, Phytotherapy Research 25: 1737-1898 (2011).
Hye Duck Choi, Yeo Kyu Youn, Wan Gyoon Shi. Positive Effects of Astaxanthin on Lipid Profiles and Oxidative Stress in Overweight. Plant Foods for Human Nutrition, 66:363–369 (2011)
Charalambos Vlachopoulos, Konstantinos Aznaouridis, Nikolaos Alexopoulos, Emmanuel EconomouIoanna, and Andreadou Christodoulos Stefanadis. Effect of Dark Chocolate on Arterial Function in Healthy Individuals American Journal of Hypertension, 18:785–791 (2005)
Shujiro B.Minami, Su-HuaSh, and JochenSchacht. Antioxidant protection in a new animal model of cisplatin-induced ototoxicity. Hearing Research 198: 137-143 (2004).
T. Rendo-Urteaga, B. Puchau, M. Chueca, M. Oyarzabal, M. C. Azcona-Sanjulián, J. A. Martínez, A. Marti. Total antioxidant capacity and oxidative stress after a 10-week dietary intervention program in obese children. European Journal of Pediatrics. Volume 173: 609–616 (2014).
Zulfitri A Mat Daud, Boniface Tubie, Marina Sheyman, Robert Osia, Judy Adams, Sharon Tubie, and Pramod Khosla. Vitamin E tocotrienol supplementation improves lipid profiles in chronic hemodialysis patients. Vasc Health Risk Manag. 9:747–761 (2013)
Constance Lay LaySawab, Anne YuqingYang, YueGuob and Ah-Ng TonyKong. Astaxanthin and omega-3 fatty acids individually and in combination protect against oxidative stress via the Nrf2–ARE pathway Food and Chemical Toxicology 62: 869-875 (2013)
Garry Duthie, Fiona Campbell , Charles Bestwick, Sylvia Stephen and Wendy Russell. Antioxidant Effectiveness of Vegetable Powders on the Lipid and Protein Oxidative Stability of Cooked Turkey Meat Patties: Implications for Health Nutrients 5: 1241-1252 (2013)
Hye Duck Choi, Yeo Kyu Youn, and Wan Gyoon Shin. Positive Effects of Astaxanthin on Lipid Profiles and Oxidative Stress in Overweight Subjects. Plant Foods for Human Nutrition 66: 363–369 (2011)