OBR carries several monoclonal antibodies that were made against ovine Prostaglandin H Synthase 1 (PGHS-1), also known as Cyclooxygenase 1 (COX-1), and are specific for COX-1 without cross reactivity to Cyclooxygenase 2 (COX-2). This antibody reacts with human, sheep, bovine, mouse and rat COX-1.
For Western blotting and Immunohistochemistry using alkaline phosphatase or horseradish peroxidase, a dilution of 1:1000 is recommended. With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
1. DeWitt, D.L., et al., Concentrations of PGH Synthase and PGI2 Synthase in the Endothelium and Smooth Muscle of Bovine Aorta. J. Clin. Invest. 72: 1882 (1983).