This antibody was prepared in rabbit using rat hematopoietic form PGD Synthase, purified on S-hexylglutathione affinity resin, as the immunogen. It is specific for hematopoietic form PGD Synthase, and does not cross react with the brain form of PGD Synthase. This antibody is useful for Western, immunohistochemistry, immunoprecipitation and ELISA applications.
Prostaglandin D2 (PGD2) is actively formed in a variety of tissues and is involved in many physiological events. PGD2, one of the major prostaglandins in the central nervous system (CNS), is involved in CNS activities, which include, induction of hypothermia and sleep, and is thought to be a new type of neuromodulator. In the CNS and other tissues of mammals, the isomerization of PGH2 to PGD2, is catalyzed by PGD synthase. Two types of PGD synthase have been reported. One is glutathione (GSH)-requiring PGD synthase; the other is GSH-independent PGD synthase. These two enzymes are very similar acidic proteins with the same molecular weight, but distinct in that they differ in amino acid composition, GSH requirement, sensitivity to 1-chloro-2,4-dinitrobenzene, and antigenic determinants.