GST Alpha ELISA
Glutathione S-Transferase (GST) has multiple isoforms. This assay is specific for Glutathione S-Transferase Alpha (GSTA) and is not known to cross react with the mu, pi, or theta variants. GSTA is a common biomarker for hepatocellular damage.1 It also conjugates GSH to 4-hydroxynonenal, a product of lipid peroxidation2 and is an important player in cellular antioxidant defense mechanisms.3
This is a standard sandwich enzyme-linked immunosorbent assay (ELISA). The plate is pre-coated with anti-GSTA and blocked, ready for the addition of samples and standards. The assay should take approximately 2.5 hours to run, plus any required sample preparation time.
When measuring urine samples, it is recommended to normalize samples to creatinine to account for variations in fluid intake and excretion. Creatinine is produced at a constant rate and can be used to normalize the concentrations of other analytes. Creatinine Assay Kit (CR01).
1. Vaubourdolle, M, et al., Clinical Chemistry 41: 1716-1719, 1995
2. Awasthi, Y. C, et al., Free Radic. Biol. Med. 37: 607–619, 2004
3. Yang, Y., et al. Toxicol. Appl. Pharmacol. 182: 105–115, 2002