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Human Plasma Lactoferrin Assay Kit

Product Overview


Human lactoferrin (LTF) is an 80 kDa glycoprotein which was first isolated from human milk. LTF is found in most body fluids and secretions, e.g., in the nose, genital tract, and tears.1 LTF in blood is secreted by neutrophils and its plasma concentration is positively related to the total pool of neutrophils and to the rate of neutrophil turnover.2 Because of its ability to strongly bind iron, LTF is considered to be bactericidal.3 In a number of cases of inflammation, LTF is released into the extracellular medium from secondary granules of neutrophilic leukocytes.4,5 Its extracellular concentration can therefore be used as an index of neutrophil activation, especially in blood samples containing anti-myeloperoxidase antibodies.

Assay Principle

This method is an enzyme-linked immunoassay (ELISA). Samples are incubated in the wells of a microplate, which have been coated with a monoclonal antibody to LTF. Any bound LTF is detected by a biotinylated monoclonal antibody to LTF. The next step of the assay is an amplification based on a biotin-avidin coupling in which avidin has been covalently linked to horseradish peroxidase. The amount of LTF is measured enzymatically upon the addition of o-phenylenediamine (OPD) at 450 nm.

Recommended References

  1. Autiero, M., Sansone, G., Abrescia, P.; (1991) J. Androl. 12:191-200
  2. Birgens. H.S.; (1985) Scand. J. Haematol. 34:326-331
  3. Baynes, R.D., Bezwoda W.R., Khan, Q.,  Mansoor. N.; (1986) Scand. J. Haematol. 36:79-84
  4. Lash, J.A., Coates, T.D., Lafuze, J., Baehner, R.L., Boxer, L.; (1983) Blood 61:885-888
  5. Kähler, S., Christophers, E., Schröder, J.M.; (1988) Brit. J. Derm. 119:289-293


Spec Sheet