Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade1. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation2. Elevated fibrinogen levels are correlated with cardiovascular disease3 and atherosclerosis4.
This is an ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of total fibrinogen levels in mouse plasma and serum. This test kit operates on the basis of sandwich ELISA where the fibrinogen binds to a capture antibody on the plate and is quantified with the use of a biotin labeled primary antibody and an avidin-HRP conjugate.
Mouse fibrinogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse fibrinogen. Color development is proportional to the concentration of fibrinogen in the samples.
- Kamath, S. and Lip, G.Y.H.; (2003) QJM 96:711-729
- Kusher, I.; (1982) Ann New York Acad Sci 389:39-48
- Kannel, W.B., et al.; (1987) J Am Med Assoc 258:1183-1186
- Hanga, K., et al.; (1989) Atherosclerosis 77:209-213
- Rezaee, F., et al.; (2002) Atherosclerosis 164:37-44