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Total Murine PAI-1 antigen assay kit

$530.00
SKU
PI93
Product Overview

Background


Plasminogen Activator Inhibitor-1 (PAI-1) is a glycoprotein and member of the serine protease inhibitor (serpin) superfamily. PAI-1 is the primary inhibitor of tissue-type plasminogen activator (tPA) and the urokinase-type plasminogen activator (uPA). This inhibition exhibits antiproteolytic properties that can lead to myocardial infarction and thromboembolic disease with elevated levels of PAI-1. Additionally, PAI-1 is thought to play a role in the function of tissue remodeling and tumor metastasis.

 

 

Assay Principle


This is an ELISA for the quantitative analysis of PAI-1 levels in biological fluid. This test kit operates on the basis of sandwich ELISA where all PAI-1 forms (complexed, latent and active) are captured and quantitated with the use of an HRP labeled secondary antibody.

The free, latent, and complexed PAI-1 all bind to the PAI-1 capture antibody coated on the well of the microtiter plate. Next, a PAI-1 primary antibody is added to bind to the captured PAI-1 on the microtiter plate. An HRP conjugated secondary antibody is then added for detection of the total PAI-1. Optimal color is reached at 10 minutes when read at 450 nm. Quantitative test results are obtained by the measure and comparison of the sample and standard absorbance readings.

 

 

Spec Sheet