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Two excellent biomarkers for inflammation are Histamine (EA31) and 15-Keto-13,14-Dihydro-PGF2α (EA20). Histamine serves as a mediator of inflammation and hypersensitivity responses and is involved in a cascade physiological effects that are influenced by typical immune response and environmental factors. 15-Keto-13,14-Dihydro-PGF2α is the major metabolite of PGFα and can be used as an unique biomarker of acute and chronic inflammation.