CYPerior enzymes are engineered ancestral P450s that exhibit improved properties such as thermal stability, solvent stability, and unique substrate and product profiles.
Each is available coexpressed with native human P450 Oxidoreductase (hCPR) or with an engineered P450 Oxidoreductase (CPRmod). These enzymes are useful for pharmaceutical lead diversification, scale up synthesis, and biocatalysis.
CYPerior CYP2ABGS is the ancestral form of the CYP2A, CYP2B, CYP2G, and CYP 2S families and is coexpressed with the modified P450 Oxidoreductase.
1. Gumulya, Y, et.al., Engineering highly functional thermostable proteins using ancestral sequence reconstruction. Nature Catalysis. 1: 878–888 (2018).
2. Harris KL, Thomson RES, Strohmaier SJ, Gumulya Y, Gillam EMJ. Determinants of thermostability in the cytochrome P450 fold. Biochim Biophys Acta Proteins Proteom. 1866:97-115 (2018).
3. Behrendorff, JBYH, &Gillam EMJ, Prospects for Applying Synthetic Biology to Toxicology: Future Opportunities and Current Limitations for the Repurposing of Cytochrome P450 Systems. Chem. Res. Toxicol. 30:453−468 (2017).